Signal Transduction — Single Cell Techniques

I Handling of Cells in Single Cell Experiments.- 1 Maintaining Cells Under the Microscope.- 2 Simple Perfusion Chambers for Single Cell Measurements.- 3 Temperature Control on the Stage of the Microscope.- 4 A Modified U-Tube Drug Application System.- 5 Laser Microsurgery as a Tool in Single Cell Research.- II Ion Channel and Membrane Potential Measurements Using the Patch-Clamp Technique.- II–1 Introduction to the Measurement Technique.- 6 Significance of Ion Channels and Membrane Potential Changes in Cells.- 7 Practical Introduction to Patch Clamping by Simulation Experiments with Simple Electrical Circuits.- 8 Improved Electrophysiological Measurements by Series Resistance Compensation.- 9 Preparation of Patchable Plant Cell Protoplasts and a Procedure for the Improvement of Gigaseal Formation.- II–2 Single Channel Measurements.- 10 Recording and Analysis of ATP-Sensitive Potassium Channels in Inside-Out Patches of Rabbit Ventricular Myocytes.- 11 Mechanotransduction and Mechanosensitive Ion Channels in Osteoblasts.- 12 The Study of (Plant) Ion Channels Reconstituted in Planar Lipid Bilayers.- 13 Ion Channel Measurement on the Cell Nucleus.- 14 Monitoring Cytosolic Calcium and Membrane Potential from Cell-Attached Patch-Clamp Currents in Human T Lymphocytes.- II–3 Whole Cell Measurements.- 15 Membrane Potential and Action Potential Measurements in Whole Cell and Perforated Patch Configurations.- 16 Perforated Patch-Clamp Technique in Heart Cells.- 17 Measurement and Analysis of Different Aspects of Potassium Currents in Human Lymphocytes.- 18 Ionic Conductances in Chicken Osteoclasts.- 19 GABAA Receptor-Mediated Chloride Currents in Acutely Dissociated Hippocampal Neurons.- 20 Measurement of Whole Cell Potassium Currents in Protoplasts from Tobacco CellSuspensions.- 21 Inward Rectifying Potassium Conductance in Barley Aleurone Protoplasts.- III Fluorescence to Measure Intracellular Ions.- III–1 Introduction to the Measurement Technique.- 22 An Introduction to the Use of Fluorescent Probes in Ratiometric Fluorescence Microscopy.- 23 How To Use Video Cameras To Acquire Images.- 24 Strategies for Studying Intracellular pH Regulation.- III–2 Flow Cytometer Measurements.- 25 An Introduction to the Working Principles of the Flow Cytometer.- 26 Flow Cytometric Membrane Potential Measurements.- 27 Flow Cytometric Measurement of Cytosolic Calcium in Lymphocytes.- III–3 Microfluorescence Measurements.- 28 Cytosolic pH and Cell Movement Measurement in Dictyostelium.- 29 Analysis of Agonist-Induced Cell Recruitment in Terms of Intracellular Calcium Mobilization in a Population of Enzymatically Dispersed Pancreatic Acinar Cells.- III–4 Confocal Microscopy.- 30 Quantitative Confocal Fluorescence Measurements in Living Tissue.- 31 Cytosolic Calcium Measurements with Confocal Microscopy.