<div>Preface…</div><div>Table of Contents…</div><div>Contributing Authors…</div><div><br></div><div>1. Overview of Mitochondrial Bioenergetics</div><div>Vitor M.C. Madeira</div><div><br></div><div>2. Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells</div><div>Ana M. Silva and Paulo J. Oliveira</div><div><br></div><div>3. High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria</div><div>Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, András T. Mészáros, and Erich Gnaiger</div><div><br></div><div>4. High-Throughput Analysis of Mitochondrial Oxygen Consumption</div><div>James Hynes, Rachel L. Swiss, and Yvonne Will</div><div><br></div><div>5. Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices</div><div>Ana Ledo, Rui M. Barbosa, and João Laranjinha</div><div><br></div><div>6. Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria</div><div>João S. Teodoro, Anabela P. Rolo, and Carlos M. Palmeira</div><div><br></div><div>7. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells</div><div>David G. Nicholls</div><div><br></div><div>8. Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production</div><div>Timea Komlódi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger</div><div><br></div><div>9. Measurement of Proton Leak in Isolated Mitochondria</div><div>Charles Affourtit, Hoi-Shan Wong, and Martin D. Brand</div><div><br></div><div>10. Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy</div><div>Sean M. Davidson and Michael R. Duchen</div><div><br></div><div>11. Mitochondrial Permeability Transition Pore and Calcium Handling</div><div>Randi J. Parks, Elizabeth Murphy, and Julia C. Liu</div><div><br></div><div>12. Redox Equivalents and Mitochondrial Bioenergetics</div><div>James R. Roede, Young-Mi Go, and Dean P. Jones</div><div><br></div><div>13. Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis</div><div>Rui A. Carvalho</div><div><br></div><div>14. Computational Modeling of Mitochondrial Function from a Systems Biology Perspective</div><div>Sonia Cortassa, Steven J. Sollott, and Miguel A. Aon</div><div><br></div><div>15. Monitoring the Mitochondrial Dynamics in Mammalian Cells</div><div>Luca Simula and Silvia Campello</div><div><br></div><div>16. Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria</div><div>Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand</div><div><br></div><div>17. Plate-Based Measurement of Respiration by Isolated Mitochondria</div><div>Shona A. Mookerjee, Casey L. Quinlan, Helen Wong, Pratiksha Dighe, and Martin D. Brand</div><br><div>18. Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells</div><div>Shun-ichi Yamashita and Tomotake Kanki</div><div><br></div><div>19. The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue</div><div>Kieran J. Clarke and Richard K. Porter</div><div><br></div><div>20. Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells</div><div>Julian Geiger and Louise T. Dalgaard</div><div><br></div><div>21. Imaging of Mitochondrial pH Using SNARF-1</div><div>Venkat K. Ramshesh and John J. Lemasters</div><div><br></div><div>22. Relation between Mitochondrial Membrane Potential and ROS Formation</div><div>Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski</div><div><br></div><div>23. Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks</div><div>Felix T. Kurz, Miguel A. Aon, Brian O’Rourke and Antonis A. Armoundas</div><div><br></div><div>24. Measurement of Mitochondrial ROS Formation</div><div>Soni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa</div><div><br></div>