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Mitochondrial Bioenergetics

Methods and Protocols

Paperback Engels 2019 2e druk 9781493992980
€ 204,99
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This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.

Specificaties

ISBN13:9781493992980
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York
Druk:2

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<div>Preface…</div><div>Table of Contents…</div><div>Contributing Authors…</div><div><br></div><div>1. Overview of Mitochondrial Bioenergetics</div><div>Vitor M.C. Madeira</div><div><br></div><div>2. Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells</div><div>Ana M. Silva and Paulo J. Oliveira</div><div><br></div><div>3. High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria</div><div>Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, András T. Mészáros, and Erich Gnaiger</div><div><br></div><div>4. High-Throughput Analysis of Mitochondrial Oxygen Consumption</div><div>James Hynes, Rachel L. Swiss, and Yvonne Will</div><div><br></div><div>5. Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices</div><div>Ana Ledo, Rui M. Barbosa, and João Laranjinha</div><div><br></div><div>6. Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria</div><div>João S. Teodoro, Anabela P. Rolo, and Carlos M. Palmeira</div><div><br></div><div>7. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells</div><div>David G. Nicholls</div><div><br></div><div>8. Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production</div><div>Timea Komlódi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger</div><div><br></div><div>9. Measurement of Proton Leak in Isolated Mitochondria</div><div>Charles Affourtit, Hoi-Shan Wong, and Martin D. Brand</div><div><br></div><div>10. Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy</div><div>Sean M. Davidson and Michael R. Duchen</div><div><br></div><div>11. Mitochondrial Permeability Transition Pore and Calcium Handling</div><div>Randi J. Parks, Elizabeth Murphy, and Julia C. Liu</div><div><br></div><div>12. Redox Equivalents and Mitochondrial Bioenergetics</div><div>James R. Roede, Young-Mi Go, and Dean P. Jones</div><div><br></div><div>13. Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis</div><div>Rui A. Carvalho</div><div><br></div><div>14. Computational Modeling of Mitochondrial Function&nbsp; from a Systems Biology Perspective</div><div>Sonia Cortassa, Steven J. Sollott, and Miguel A. Aon</div><div><br></div><div>15. Monitoring the Mitochondrial Dynamics in Mammalian Cells</div><div>Luca Simula and Silvia Campello</div><div><br></div><div>16. Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria</div><div>Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand</div><div><br></div><div>17. Plate-Based Measurement of Respiration by Isolated Mitochondria</div><div>Shona A. Mookerjee, Casey L. Quinlan, Helen Wong, Pratiksha Dighe, and Martin D. Brand</div><br><div>18. Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells</div><div>Shun-ichi Yamashita and Tomotake Kanki</div><div><br></div><div>19. The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue</div><div>Kieran J. Clarke and Richard K. Porter</div><div><br></div><div>20. Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells</div><div>Julian Geiger and Louise T. Dalgaard</div><div><br></div><div>21. Imaging of Mitochondrial pH Using SNARF-1</div><div>Venkat K. Ramshesh and John J. Lemasters</div><div><br></div><div>22. Relation between Mitochondrial Membrane Potential and ROS Formation</div><div>Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski</div><div><br></div><div>23. Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks</div><div>Felix T. Kurz, Miguel A. Aon, Brian O’Rourke and Antonis A. Armoundas</div><div><br></div><div>24. Measurement of Mitochondrial ROS Formation</div><div>Soni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa</div><div><br></div>

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